Dear Dr. Zucker, Annexin V staining is compatible with fixation/permeabilisation of cells as long as the fixative/perm reagents have Ca/Mg present. G. Hodge et al, (1999) Journal of Immunological Methods, 225, 27-38. Regards, Greg Hodge (hodgeg@mail.wch.sa.gov.au) > ---------- > From: Leonid Volkov > Sent: Saturday, 18 March 2000 3:34 > To: Cytometry Mailing List > Subject: Re: viability on fixed cells > > > Dear Dr. Zucker, > > >Is it possible to measure viability in a cell population and then fix the > >cells prior to analysis by flow cytometry. > [...] > > Does any one have any knowledge or information on this type of protocol. > > Levelt CN, et al. Streptavidin-tricolor is a reliable marker for nonviable > cells subjected to permeabilization or fixation. Cytometry. 1994 Jan > 1;15(1):84-6. > > I hope this information is helpful > > Best regards > > > Dr. L. VOLKOV > Ass. en Cytometrie -Microscopie > Serv. d'Immunologie > Fac. de medecine > 3001, 12 av.Nord SHERBROOKE (Quebec); CANADA > J1H 5N4 > Tel: (819) 346-1110 ext 4867 > Fax: (819) 564-5215 > Leonid Volkov <leonidv@crccuse.usherb.ca> >
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