Bob Zucker asked about the cell viability assay in which dead cells can be detected after fixation. Indeed, Allan Pollack developed the method combining Hoechst dye with PI which can be used on live cells, which then can be fixed and measured later (Pollack & Ciancio, Meth.Cell Biol. (Flow Cytometry), Vol 33, 19-24, 1990. We were using this method and its modification is published in Meth.Cell Biol. (Flow Cytometry 2nd Edition) Vol 41, 15-38, 1994. Zbigniew Darzynkiewicz
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