>OK Flow JO is great ANALYSIS software, for most things....but it still will >not Acquire data, I'm not really sure that is the point here. Haven't we been talking about ANALYSIS? Most, if not all, the people who have been having problems have bought new G4 machines for post-acqusition analysis (and other uses). Those who simply upgraded to OS 9.0 could easily downgrade to OS8.6 when it didn't work - it was those people with machines that only run OS9.0 that were stuffed (by Apple and by BD) If you bought one for acquisition then I agree wholeheartedly that you should consider it an object lesson (esp given *BDs* record (AND their recommendations) as well as Apple's) > allow back gating of populations Excuse me? >and is not the easiest >for "Graphical" Analysis... That is entirely subjective (and I'm not even sure what you mean by "graphical" analysis!). As I have said before I find that complete novices pick up FlowJo MUCH more quickly than CellQuest. >so the mantra of "dumping" Cellquest and moving >to FlowJo is over simplistic at best and is only a solution for those who >never touch the FACS their data comes from in the first place. Not true either. I touch the flow cytometer while it collects all my data AND I always use FlowJo for my analysis. No, FlowJo is not a solution for acquisiton but again I don't think that is what we have been talking about. FlowJo has been an excellent solution for us and we do all our own acqusition (using CellQuest on an older machine with an older OS - as you said, if it ain't broke...). Our data is of such a nature that ANY increase in machine speed directly translates to increased analysis throughput - FlowJo has allowed us to take advantage of those increases in speed. Analysis takes MUCH longer the acqusition in our experiments and acqusition doesn't require any where near the same computing power. We were even using FlowJo while the cytometer was still running from an HP - CellQuest nowhere in the loop! I think acquisition and analysis are two separate tasks and it makes sense to use the best tools for each one. Most people need two computers, and hence two software licenses, anyway... I don't think I was being "simplistic" in advocating dropping CellQuest :) ( but I should have made it clearer I was talking about ANALYSIS only) >And it still >isn't available for the PC platform, so once again, it is not a cure-all. Neither is CellQuest last time I looked!
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:11 EST