Hi Another quite probable explanation is that the CD19/CD14 positive "cells" are doublets or triplets containing both monocytes and B-Cells. You can try to sort then onto microscope slides and counterstain with PI then view with a fluorescence mic but such clumps may dissociate during sorting. I've seen this with T-Cells and monocytes. Simon I was wondering if anyone has seen a population positive for both CD14 and CD19. I am staining peripheral blood lymphocytes and am looking at the B cell population. I originally just used CD19 to get the B cells. However recently I began using CD3, CD14 to exclude any T cells and monocytes. I will use CD56 as well to exclude NK cells. However, I did see some cells that were positive for both CD19 and CD14. Are these cells monocytes or are they B cells. I do want to look at all B cells, my question is how can I find out if these cells are truly B cells or if they are monocytes? Thanks, Annuska Glas Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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