I'm using an immunofluorescence procedure and microscope to count field samples of picoplankton cells, 2-3 um diameter, (Aureococcus anophagefferens "brown tide"). Clumping of cells or embedding of cells in detritus can result in a big error. I've tried several detergents to separate the cells; Aquet at 2% is best so far; gentle vortex mixing in combination with detergent also helps. Aureococcus excretes mucopolysaccharide. I'd appreciate advice on separation of the glutaraldehyde-fixed but relatively delicate cells. Thank you, John Mahoney
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