Hello - Does anyone know if rat neutrophils release L-selectin when stimulated with fMLP? We've treated LongEvans rat blood with fMLP (uM) and we readily detect Mac1 upregulation and neutrophil degranulation, but L-selectin (detected with HRL-1 antibody) does not get shed. Under similar conditions, human neutrophils respond enthusiastically and release their L-selectin. We can get shedding with A23187 etc, but not fMLP. Any ideas? Thank you, Snezna New Mexico Tech
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