A subset of NK cells express low levels of CD8. There are 2 expression levels of CD56 (bright and brighter) on NK cells. The CD56 bright cells don't express the KIR receptors (Joe Phillips, DNAX, personal communication) but otherwise are normal NK cells. tessa kerre wrote: > Dear flowers, > > I posted this message some time ago, together with the proposal to send > anyone interested a CFSE summary. I got a huge amount of people wanting > the summary, but nobody answered my question in the second part. My > theory is that people got so enthusiastic about my proposal they didn't > look further in the e-mail. > > So I am sending my questions again, hopin someone would be able to > anwser them... > > I have some questions regarding the phenotype of CB cells. > > I deplete the fresh or thawed frozen mononuclear cell fraction of human > cord blood , and deplete (with magnetic beads) for CD3. I have done some > flowcytometrical analysis on these cells, and I have some questions: > > - I see a CD4low population (monocytes) and a reminescent CD4high, but I > see the same phenomenon with CD8 (sometimes there's a nice segregation > between the two, but in most cases there isn't): are the CD8low cells NK > cells? > > - In the CD56+ population I also observe 2 intensities: does anyone know > what these two populations represent? Is it possible that the CD56high > cells are gamma-delta cells? > > Kind regards, > > Tessa > > Tessa Kerre, MD > Gent University Hospital > Belgium
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