Jackie, I am routinely analyzing and sorting very small bacteria (down to 0.2 micron diameter) from natural water samples using DAPI, Syto13 an other stains. Usually, I trigger on fluorescence in order not to lose the smaller cells. This gets more important when sorting, as the drop drive may disturb scatter signals. Besides that, we employ a special trigger board on our MoFlo that enables us to trigger on logarithmically amplified signals and logical combinations (AND/OR) of different signals. However, measuring quite "large" bacteria from cultures, should be easily possible without. For Alignment and counting reference I am using Molecular Probes Alignflow 2.5 micron which are available for 488nm and UV-excitation and 1.0 micron TransFluoSpheres (also Molecular Probes). Good luck Stefan ___________________________________________________________ Stefan Andreatta University of Innsbruck, Institute of Zoology and Limnology Technikerstrasse 25, A-6020 Innsbruck, Austria phone:++43-512-507-6122; fax:++43-512-507-2930 http://zoology.uibk.ac.at/limno/ > > Dear Flowers, > > I will be needing to sort bacteria soon. I've never sorted bacteria before > so any hints, suggestions, > advice are welcome. Also, could someone recommend alignment beads for > bacterial sorting. My > instrument is a FacsVantage. Thanks in advance for your help. > > Jackie Saleh > Aventis Pharmaceuticals > > > >
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