Hi there, flow aficionados. I know we've all addressed the post-sort viability (after high-speed sorting) question many times over. What I am looking for is not "whether" but "how much," so to speak. To that end, I'd like to ask the List if anyone knows of any published work that has addressed the question not of whether or not cells are viable after such sorting (we know they are, or can be) but "how" viable, that is, do they produce less cytokine or other product, proliferate a little more slowly, or survive for less time, etc., etc. under the same conditions versus "low-speed" sorted cells? Shucks, references that just address this question, if it is not the primary objective of the work, would be great to have. If anyone is willing to share their unpublished insights, I'd love to hear them as well. Thank you very much, Cliff McArthur University of California at San Francisco Departments of Medicine and Immunology/Microbiology 415-502-6860 ____________________________________________________________________ Get your own FREE, personal Netscape WebMail account today at http://webmail.netscape.com.
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