Dear flowers, I have a question regarding cell suspensions:is there anyone out there who has experience in making cell suspensions of murine liver and lung? I can get cells out of these organs without any problem using a tissue grainer and delicate use of a scalpel, but now I would like to calculate absolute numbers of cells present in these organs, so I have to get the most cells possible out of them. Do you know of a method that would give a high yield by destructing all connecting tissue, without being toxic for lose cells? Kind regards, Tessa Tessa Kerre, MD Gent University Hospital Belgium
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