Kudos to Jim Houston. It is all in the needs of the laboratory. "Houston, Jim " <Jim.Houston@stjude.org> on 02/28/2000 09:38:41 AM To: cyto-inbox cc: Subject: RE: high speed sorter: sorting and analysis To All: I believe this is suppose to be a none commercial exchange of information. This response sounds like a commercial to me. To my knowledge no other sales persons for instruments offers these suggestions. I have no problem in listening to the high end use of the MoFlo. Let the users be heard without company interventions. I understand that some feel the MoFlo is like the Porsche of the Flow Sorter world, but you would not take a Porsche off road where you need a Four wheel drive. Jim Houston Coordinator, Flow Cytometry and Sorting -----Original Message----- From: Dr Robert Ashcroft [mailto:cytomat@netcore.com.au] Sent: Friday, February 25, 2000 5:47 AM To: cyto-inbox Subject: RE: high speed sorter: sorting and analysis Dear Andreas, Surely there is a message to you and all of us in the nature of the replies to your earlier requests, where you say "almost only MoFlo users answered" In my simple view, "Winners like to trumpet their success, and runners up stay silent". Is it different for you? Let's ask the List again, why is no-one else responding? On the MoFlo, there is no difference to analyzing and sorting except for a simple tick you add to the software. But you can analyze faster once the flow rate goes over 40,000/s, except if you are enrich sorting; but that's another question. Let's refine Andreas' questions, SORTING Who routinely sorts cells at flow rates above 20,000 cells/s on signals from two lasers? Declare yourselves, and tell us what your REPRODUCIBLE RESULTS ARE. Finally, are you sorting 2-Way or 4-Way? ANALYZING #1 Who routinely analyzes cells at input rates above 20,000/s on signals from two lasers? #2 Who routinely or sometimes analyzes cells at input rates above 50,000/s on signals from 2 lasers? I await the judgment of the people. Bob Ashcroft -----Original Message----- From: Andreas Simm [mailto:simm@klin-biochem.uni-wuerzburg.de] Sent: Wednesday, 23 February 2000 18:38 To: cyto-inbox Subject: high speed sorter: sorting and analysis Hello everybody, I know that this question appears nearly all three month, but I now have to decide which high speed sorter we should buy. As we will have only one apparatus for both, analysis and sorting, the decision (may be) will not be the same as if one consider to buy a flow cytometer for sorting purposes only. And as far as I know, almost only MoFlo user answered to the last queries. So my questions: What is the experience with the Altra (Coulter)? I did not hear from any experience in this list. What is the experience with the FACS-Vantage? >From the last queries, the MoFlo tends to be the best sorter (but only users of the MoFlo answered!). What's the experience with it for analysis purposes only. Is it easy to handle? Is anybody in the flow community, who can really compare these sorters? Thanks for every statement in advance! Andreas My address: PD Dr. Andreas Simm Institut fuer Klinische Biochemie und Pathobiochemie Versbacher Str. 5 D-97078 Wuerzburg Germany Tel.: +49 931-201-3144 FAX.: +49 931-201-3153 E-mail: simm@klin-biochem.uni-wuerzburg.de
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