Bob I sort using 2 lasers/FL1,Fl2,Fl3,Fl4, At >25K/second, 2ways---- but 4ways sorts when they are needed, AND I would rather drive my porche(MoFlo) than any offroad and slow 4 wheel drive. If you had DSL--- would you still hook-up your 56K modem? Dom Fenoglio On Mon, 28 Feb 2000, Houston, Jim wrote: > Date: Mon, 28 Feb 2000 09:38:41 -0600 > From: "Houston, Jim " <Jim.Houston@stjude.org> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: RE: high speed sorter: sorting and analysis > > > To All: > > I believe this is suppose to be a none commercial exchange of information. > This response sounds like a commercial to me. > To my knowledge no other sales persons for instruments offers these > suggestions. > > I have no problem in listening to the high end use of the MoFlo. Let the > users be heard without company interventions. > > I understand that some feel the MoFlo is like the Porsche of the Flow > Sorter world, but you would not take a Porsche off road where you need a > Four wheel drive. > > Jim Houston > Coordinator, Flow Cytometry and Sorting > > > > > -----Original Message----- > From: Dr Robert Ashcroft [mailto:cytomat@netcore.com.au] > Sent: Friday, February 25, 2000 5:47 AM > To: cyto-inbox > Subject: RE: high speed sorter: sorting and analysis > > > > Dear Andreas, > Surely there is a message to you and all of us in the nature of the > replies > to your earlier requests, where you say "almost only MoFlo users answered" > In my simple view, "Winners like to trumpet their success, and runners up > stay silent". > Is it different for you? > > Let's ask the List again, why is no-one else responding? > On the MoFlo, there is no difference to analyzing and sorting except for a > simple tick you add to the software. But you can analyze faster once the > flow rate goes over 40,000/s, except if you are enrich sorting; but that's > another question. > > Let's refine Andreas' questions, > > SORTING > Who routinely sorts cells at flow rates above 20,000 cells/s on signals > from > two lasers? > Declare yourselves, and tell us what your REPRODUCIBLE RESULTS ARE. > Finally, are you sorting 2-Way or 4-Way? > > ANALYZING > #1 Who routinely analyzes cells at input rates above 20,000/s on signals > from two lasers? > #2 Who routinely or sometimes analyzes cells at input rates above 50,000/s > on signals from 2 lasers? > > I await the judgment of the people. > Bob Ashcroft > > > > > -----Original Message----- > From: Andreas Simm [mailto:simm@klin-biochem.uni-wuerzburg.de] > Sent: Wednesday, 23 February 2000 18:38 > To: Cytometry Mailing List > Subject: high speed sorter: sorting and analysis > > > Hello everybody, > I know that this question appears nearly all three month, > but I now have to decide which high speed sorter we > should buy. As we will have only one apparatus for both, > analysis and sorting, the decision (may be) will not be the > same as if one consider to buy a flow cytometer for > sorting purposes only. > > And as far as I know, almost only MoFlo user answered > to the last queries. > So my questions: > What is the experience with the Altra (Coulter)? I did > not hear from any experience in this list. > What is the experience with the FACS-Vantage? > >From the last queries, the MoFlo tends to be the best > sorter (but only users of the MoFlo answered!). What's the > experience with it for analysis purposes only. Is it easy to handle? > Is anybody in the flow community, who can really > compare these sorters? > > Thanks for every statement in advance! > > Andreas > > > > > My address: > > PD Dr. Andreas Simm > Institut fuer Klinische Biochemie und Pathobiochemie > Versbacher Str. 5 > D-97078 Wuerzburg > Germany > > Tel.: +49 931-201-3144 > FAX.: +49 931-201-3153 > E-mail: simm@klin-biochem.uni-wuerzburg.de >
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