Hi Maryalice, Yes, we routinely obtain cell suspensions for flow cytometry by mincing skin biopsies with a scalpel and eluting the resulting suspension through a fine mesh filter. The resulting cell count is often not high and there often are many disrupted cells, but we have been successful with this approach on quite a few cases, usually cutaneous T cell lymphomas or B cell lymphomas not ATLL. -- Brent Wood MD PhD Associate Director of Hematology Laboratory University of Washington Medical Center EM: woodbl@u.washington.edu Phone: (206) 548-6199 Fax: (206) 548-6189 ---------- >From: owner-cytometry@flowcyt.cyto.purdue.edu >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Date: Thu, Feb 24, 2000, 7:10 AM > > > Has any one gotten good cell recovery with surface antigens intact > from skin biopsies? The cells are adult T cell leukemia/lymphoma > cells and are a little fragile. We tried mincing and just got debris. > We are thinking of aspirating the lesion. Any bright ideas? > > > Maryalice > Maryalice Stetler-Stevenson > Director Flow Cytometry Unit > Laboratory of Pathology, NCI, NIH >
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