Dear flowers, First of all, a big thanks for all people who were so kind to take the time to answer my questions on CFSE. I put all the information together (anyone who is interested can receive a copy), and some preliminary experiments show that the advice helped a lot! Thanks! I have some questions regarding the phenotype of CB cells. I deplete the fresh or thawed frozen mononuclear cell fraction of human cord blood , and deplete (with magnetic beads) for CD3. I have done some flowcytometrical analysis on these cells, and I have some questions: - I see a CD4low population (monocytes) and a reminescent CD4high, but I see the same phenomenon with CD8 (sometimes there's a nice segregation between the two, but in most cases there isn't): are the CD8low cells NK cells? - In the CD56+ population I also observe 2 intesities: does anyone know what these two populations represent? Is it possible that the CD56high cells are gamma-delta cells? Kind regards, Tessa Tessa Kerre, MD Gent University Hospital Belgium
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