Hello out there, One of the users of our facility has a concern regarding the advisability of diluting a particular antibody. Since these reagents are quite expensive she doesn't want to go ahead assuming that all will be well. Please respond to her following question directly at: sandra.aswald@utoronto.ca Thanks I am planning to use the antibody: anti-human IL-12 (p40/p70) mouse IgG FITC; cat.# 18994A from Pharmingen. It should be used at aprox. 0.5ug per 10 million cells, and comes as 0.1mg in a total volume of 200ul. I was wondering a) use only 1ul undiluted AB per 10 million cells b) dilute 1:10 and use 10ul Question: .Since I will be doing intracellular FACS with permeabilized cells, is it O.K. to dilute the antibody? .If I can dilute it, is there an alternative to using the Pharmingen Perm/Wash (TM) buffer? This is the only thing that Pharmingen suggests diluting their anti-cytokine AB's with. Thanks for your help Sandra
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