Dear Group, I am using Caltag's anti-CD4 (murine) antibodies. When I use the PE conjugated AB, I get 2 populations, nice separation. When I use the identical AB conjugated to TC, however, on the same sample, I get 3 populations with a new, lower intensity population. This lower intensity population is not eliminated by blocking with anti-CD16/32 ABs. It appears to be a separate population since the total # of + cells increases by the amount of the new population. Has anyone seen this with TC? Or is this a Caltag quality control issue? Many thanks for comments. Bill Ridgway
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