Cell activation for chemokine production

From: Othoro, Caroline (COthoro@kisian.mimcom.net)
Date: Thu Feb 17 2000 - 09:57:05 EST


Hello all,

Our lab has no experience in the measurement of chemokines by flow
cytometry.  We would like to do so for RANTES, MIP-1 alpha and IP-10.  We
have used the recommended protocal and reagents from Pharmingen,  with not
much success.  Does anyone have a protocal that's sure to work.

Thanks

Carol




Caroline Othoro Watta
CDC-KEMRI
P.O. Box 1578
Kisumu, Kenya

Tel:+254-35-22929
Fax:+254-35-22981



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