In <URL:news:local.facs> on Tue 15 Feb, Marc Langweiler wrote:
>
> Greetings,
>
>
> Is anyone familiar with use of anti-CDw52 for flow cytometric assessment of
> NHL's? Cursory examination of the literature reveals reports of its usage of
> several clones utilizing indirect staining IHC.
>
> How about availability of a conjugated reagent? I note that BSR has a rat IgM
> FITC conjugate, but this appears to be designed for IHC on cryostat sections.
Serotec Inc (800-265-7376) have rIgG2b CD52 FITC or RPE
I have no experience of it in paraffin sections but if there are published
reports... It certainly works in flow cytometry.
Best wishes
Nick
--
Nick Holmes Division of Immunology, Dept of Pathology
Cambridge University, Cambridge CB2 1QP, UK
Tel:+44 1223 333871 Fax:+44 1223 333875
mailto:nh106@mole.bio.cam.ac.uk http://www.path.cam.ac.uk/~nh106/
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