dissociating ligands from receptors

From: Becky Kelly (Eak@medicine.wisc.edu)
Date: Wed Feb 16 2000 - 17:17:32 EST 

I am measuring cell surface receptors for cytokines.  How do I know 
if a decrease in fluorescence is due to modulation of  receptor or to 
masking of the receptor by cytokines that are acquired in vivo?  I 
have tried, unsuccessfully,  to detect surface-bound cytokine before 
and after incubating cells with cytokine.  Is there a way to 
dissociate ligand-receptor complexes without altering the receptor in 
the process?

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