Having recently acquired some HL60's from ATCC I was wondering if anyone else is getting a rather high fluorescence in the FL3(long red) channel? Are the cells scavenging the Phenol red from the Media? (Gibco IMDM) -----Original Message----- From: Oscar Kai [mailto:Okai@kilifi.mimcom.net] Sent: Monday, February 14, 2000 2:17 AM To: cyto-inbox Subject: HL60 cell line Dear flowers, This might not be a direct flow question, but needed your help. l have started the use of HL60 cells which l induce with N,N, DMF(Dimethyl formamide) to make them mature. my question then is what molecular switch is put on the cells that make them progress from the dividing cells to become mature cells? ANY HELP? If possible could one furnish me with references too. OSCAR K. KAI KEMRI(CGMRC)/WTRL IMMUNOLOGY DEPT P.O BOX 230 KILIFI KENYA Tel. 254 0125 22063 or 22324 Fax. 254 0125 22390 email:Okai@kilifi.mimcom.net
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