> ltalbot@rdg.boehringer-ingelheim.com wrote:
> >
> >> I have a basic question. I am receiving Rat and Dog blood in 3 ml vacutainer
> > tubes with EDTA. We are looking at basic cell surface markers. If I
> > receive blood on a Friday, how can I preserve the blood to analyze on
> > Monday? Can I leave it in the tube it was drawn in? Should I take it out
> > of the vacutainer and preserve it another way? Should I stain and fix it
> > for later analysis? And would preservation be best at room temp or 4*C?
>
The guidelines for human whole blood samples suggest storage at
room
temperature and prep within 30 hours. However, your best results will
come from sampels prepped immediately [or at least the same day]. I
would recommend labeling with monoclonals and fixing the cells on the
day they arrive. Once fixed, at least for human samples, they;re
stable
for quite a while. My thinking is that once drawn, all those cells are
dying and the sooner you assay them the better.
Good luck,
Tom
--
*****************************************************************************
Thomas W. Mc Closkey, Ph. D., Director of Flow Cytometry
North Shore University Hospital, New York University School of Medicine
Boas Marks Biomedical Research Center, 350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866
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