Hello Kathi, I think you can buy 96 well plates which have filters at the bottom of each well, and you can wash fixed cells in these units using a vacuum apparatus. I can't come up with the manufacturer names, but I would think that a few calls to the major plate manufactures (Falcon, Costar, Corning, Greiner) or even a distributor like Fisher or VWR might help in locating the plates. I did something similar in the past, using a more macro setup, but the reference might be useful to you: A protocol fo Papanicolaou staining of cytologic specimens following flow analysis Cytometry 7:101-103 (1986) Peter Lopez > ---------- > From: Kellar, Kathryn L. (Kathi)[SMTP:klk1@CDC.GOV] > Sent: Thursday, February 10, 2000 10:39 AM > To: Cytometry Mailing List > Subject: Inguiry to be sent > > > Please post this inquiry to the bulletin board: > > A collaborator is searching for a filtration method or other means of > washing many samples of fixed cells to be analyzed by flow cytometry. If > you know of such a method, hopefully in a 96-well format, please let me > know. > > Kathi Kellar, PhD > National Center for Infectious Diseases > CDC > 1600 Clifton Rd, NE > Atlanta, GA 30333 > 404-639-1419 > FAX 404-639-3129 > klk1@cdc.gov > >
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