Pig mononuclear leukocyte density

From: G Robin Barclay (Robin_Barclay@compuserve.com)
Date: Wed Feb 09 2000 - 18:47:05 EST


Dear colleagues
This question does not relate directly to flow cytometry - but there is
probably someone out there who has the information.  A colleague is
interested in isolating mononuclear cells, including stem cells, from pig
bone marrow.  Using standard ficoll-hypaque intended for human mononuclear
cell isolation his yields are poor compared to human bone marrow.  What is
the optimal f-h density for isolation of pig mononuclears?  Will pig stem
cells respond to any of the human recombinant cytokines (e.g. for colony
formation)?
Robin Barclay
SNBTS Edinburgh



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