We would be very interested in doing this too. If anyone knows whether or how you can fix cells, sort and then isolate "good" RNA, please let the list know. Thanks, Mark Shlomchik, MD, PhD Associate Professor of Laboratory Medicine and Immunobiology Yale University School of Medicine mark.shlomchik@yale.edu At 4:31 PM -0400 1/20/00, Meral Keskintepe wrote: >Dear group, > >One of our customer wants to sort fixed monocytes to extract RNA. He wants >to use % 4-5 formaldehyde to fix the cells. Is that a good choice to >protect RNA? and what is the best collection media for this purpose. Any >comment would be appreciated. Thank you very much inadvence. > >Meral. > >------------------------------------------------------------------------------ > >Meral Keskintepe, PhD. > >Institute of Molecular Medicine and Genetics >Medical College of Georgia >15th street CA-2022 >Augusta, GA. 30912-2600 > >Tel: (706) 721-8880 >Fax: (706) 721-8732 > >---------------------------------------------------------------------------- >-- Mark Shlomchik, MD, PhD Associate Professor of Laboratory Medicine and Immunobiology Yale University School of Medicine 203-688-2089 203-688-2748 (fax) mark.shlomchik@yale.edu
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:06 EST