Does anyone have experience in performing analysis of red cells in whole blood. I am encountering problems with the amount of autofluorescence associated with whole blood samples. When I look at FL1 VS FL2 plots of "Cells Only", the majority of cells are in the lower left quadrant. There are some cells that are found at a 45 degree angle to the lower left quadrant. It reminds me of a "comet". The problem is that the autofluorescence is interfering with stained cells that are double positives. In order to complicate matters, we are looking for 50 events out of 50,000 in the whole blood ( 0.1%). How is this type of problem usually solved ? I tried using trypan blue but there was only a minor reduction in autofluorescence. I came across crystal violet as a quencher but fixation of the whole blood is not an option. thanks in advance for your responses, Steve
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:06 EST