The following message was originally sent to Gang Chen in response to his thanking people who responded to his message entitled "Ask for help". He was asking for help using CFSE on lymphocytes. Although I sent the message twice, originally on 1/28/99, I never got a response. So, I have decided to go to the source of his help. Namely, the correspondents to Cytometry e-mail. I hope that those who helped him can also help me. I didn't mention in my original message that I found concentrations of CFSE as low as 1 ug/ml to be apparently highly toxic to the cell lines when used for 10 minutes in the original loading of the cells. Kevin G. Waddick, Ph.D. Parker Hughes Institute 2657 Patton Road St. Paul, MN 55113 (651) 628-9988 x 224 Kevin Waddick wrote: > I don't know why, but I haven't picked up on the exchange of messages > regarding CFSE that you are thanking people for. I have been trying to > use CFSE without success to analyze cell divisions. We have used cell > lines from the B cell (Nalm-6 and Keller-2) and myeloid (HL-60) lineages > and do not get any separable peaks that would reliably correspond to > cell divisions. Is CFSE only useful in tracking cell divisions when > starting with primary cells taken from a person or animal that are then > prompted to enter cycle from the resting state? > I hope the responses you got can help me solve the problem I am > having when trying to track divisions of cell line cells. Thanks for any > help you can provide me. > > Dr. Kevin G. Waddick > Hughes Institute > 2657 Patton Road > St. Paul, MN 55113 > (651) 628-9988 x 224
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