Doug: I'd just like to clarify something from Jose Carlos' post. The StemCell Technologies product/Kit (StemSep) he is referring to consists of a cocktail of lineage specific antibodies (biotinylated), crosslinking reagent, and magnetic particles to enrich mouse progenitor cells from bone marrow. This is a negative selection system where the unwanted mature lineage committed cells are labeled with antibody/magnetic particles and removed. This pre-enrichment system works well with cells that are going to go on to a flow cytometry step because the recovered cells have not been labeled with antibody (so you are not limited in your FACS staining choices). Although this cocktail was designed for immunomagnetic cell separation, it can also be easily used as a pre-mixed cocktail of lineage-specific antibodies for flow work. The antibodies in the cocktail (anti-CD5, anti-CD45R, anti-CD11b, Gr-1 and TER119) are all biotylated and supplied as a cocktail in their own vial at a concentration suited to staining for flow cytometry. If you have any further questions about how this pre-mixed cocktail can be used for your experiments, you can contact StemCell's technical support or write directly to me. ............................................................................... Jane Yui, PhD Assistant Marketing Manager StemCell Technologies, Inc. 777 West Broadway, Suite 808 Vancouver, BC Canada V5Z 4J7 Phone: (604) 668-0858 Fax: (604) 877-0704 http://www.stemcell.com ____________________Reply Separator____________________ Subject: Re: Lin cocktail Author: <fharrod@PharMingen.com > Date: 1/27/00 9:12 AM BD PharMingen now has a "Mouse Lineage Panel" of five pre-diluted biotin conjugates. They are the same mAbs that are commonly used in the lineage "cocktails" for stem cell purification. The set should be available mid-February. For more information, you may contact BD PharMingen or reply directly to me. Florence Harrod Program Manager, Research Immunocytometry BD PharMingen "Jose C. Segovia" <segovia@CIEMAT.ES> on 01/25/2000 11:14:21 AM To: cyto-inbox cc: (bcc: Florence Harrod/SDCA/BDX) Subject: Lin cocktail Dear Douglas, Miltenyi-Biotec has this kind of cocktail in a kit for purification of LinSca-1+ cells. But I don't know if they sell the cocktail alone. You can ask them. And Stem Cell Technologies also have it but again in a kit. I hope this helps. Jose Carlos >We have heard that there is a pre-mixed lineage cocktail for mouse flow >work, but no one can tell me the name of the company that manufactures it. >......has anyone heard of this, and what company makes it. > >Thx. > >Doug > >------------------------------------------ >Douglas Olson >AIDS Research Center >Massachusetts General Hospital >149 13th Street >Boston, MA 02129 >(617)724-2660 - Phone >(617)726-4691 - Fax >Dolson3@bics.bwh.harvard.edu - Email > > > Jose C. Segovia. PhD Dpt. of Molecular and Cellular Biology CIEMAT Av. Complutense, 22 28040 Madrid SPAIN Tfn : 34 91 346 6484 Fax : 34 91 346 6393 E-mail : segovia@ciemat.es
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:05 EST