Jacqueline- we've done the following with great success, and it does not require a special kit or reagent: buffy coat the blood, extract wbc layer ficoll the wbc layer keep pellet lyse pellet, wash stain to check for purity (cd15) - our samples have always been in excess of 99% pure granulocytes. hope this helps. do ----------------------------------- Douglas Olson AIDS Research Center Massachusetts General Hospital 149 13th Street Boston, MA 02129 (617)724-2660 - Phone (617)726-4691 - Fax Dolson4@partners.org > -----Original Message----- > From: Jacqueline Saleh [SMTP:jacqueline.saleh@ariad.com] > Sent: Friday, January 28, 2000 3:15 PM > To: Cytometry Mailing List > Subject: granulocyte > > > Dear Flowers: > > Can anyone give me a protocol for isolation of granulocytes? Thanks in > advance. > > Jackie Saleh > Aventis Pharmaceuticals > >
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