Post-fixation staining

From: tarnok@medizin.uni-leipzig.de
Date: Tue Feb 01 2000 - 15:14:43 EST


Hi all,

how can we stain CD antigens on cells fixed with acetone? We have tried
several antibodies for post-fixation (e.g. anti CD3, 4, 8, 14) with
virtually no specific fluorescence signal.

Thanks for any help

Attila



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