Dear Flowers,I have recently started using CloneCyt on a FacsVantage.We have benn sorting single cells into a 96 well plate which then has PCR. Cells are usually detected in half the wells.When I sort 5 cells onto a lid I can usually find 5 cells in most positions(by dissecting microscope), finding single cells is a lot harder. I intend to try again using blue stained cells.What sort of success do other people get any ideas how I can check/improve it Thanks Geoff Morgan Babraham, cambridge U.K. geoff.morgan@bbsrc.ac.uk
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