We are not seeing a difference between FC gamma RII/III blocked and unblocked samples for flow analysis of mouse bone marrow or mouse splenocytes. We are also not seeing high background in our histograms. For example, when looking at CD4 in splenocytes we see a tightly grouped negative population in the first decade on the log scale with an even tighter CD4 (36%) positive population in the third decade. No broad spread between them to speak of. In the Pharmingen catalogue they show an assay for their Fcgamma-block that shows a broad peak showing 100% staining for Thy1.2 in the absence of blocking. Any ideas why we see no effect? We have achieved similar results with Ly-6G x CD11b, Ter119 x CD44 and B220 x IgM - absolutely no difference between FC blocked or not blocked. Why do we keep seeing references to blocking FC receptors in the literature when we don't seem to be having a problem with background to begin with? Are we doing something wrong? - Derek Schulze Flow Cytometry and Confocal Microscopy Core Facility Cancer Research Labs Queen's University Kingston, Ontario Canada
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