Dear Flow-ers, I would like to thank everybody who helped in this case. As to our possibilities, we performed these stainings: surface CD21 (0%) surface CD22 (84%) surface kappa-chain (1.2%) surface lambda-chain (2.3%) surface CD30 (0%) surface CD38 (87%) intracellular CD22 (75%) intracellular kappa-chain (0.6%) intracellular lambda-chain (97%) intracellular CD3 (6.7%) Based on these findings, we suppose that this is an immature B-cell leukaemia. Thank you again for the quick responses, Best regards, Julia ------- Forwarded Message Follows ------- From: Self <SOTE-NET/SZEBJUL> To: cyto-inbox Subject: CD19-/CD10+/HLA-DR+ cells? Date: Mon, 24 Jan 2000 16:06:53 GMT+100 Dear Flow-ers, We got a bone marrow sample from a 5-year-old boy who has anaemia, thrombopenia and leukopenia, returning febrile status, leg pain. We found that only 10% of bone marrow cells expressed CD45. 90% of the blast-like cells were HLA-DR+ and CD10+, but they do not express CD19. The percentage of CD34+ cells are also very low (0.1%). We could not detect neither CD33 or CD13. 5% of the cells were positive for CD3, CD5 or C7. What kind of malignancy can this be? I would appreciate any comments. Julia Julia B. Szeberenyi M.D Dept. of Genetics, Cell-and Immunobiology Laboratory of Flow Cytometry Semmelweis University of Medicine H-1089 Budapest, Nagyvarad ter 4 Hungary email: szebjul@net.sote.hu fax:+36-1-303-69-68
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:04 EST