Anne,
Don't know for sure if this will help but when I was running the
lab in Oman I used the ReticOne dye from Coulter which uses a
metachromatic dye (Chlorophosphine O - I think), staining both DNA and RNA
but fluorescing different colours. It's designed for use on the XL and, if
memory serves correct, is set up on FL1 & FL3.
I did run some P Falciparum infected RBC's on it and had some slight
indication that they could be seen but the parasitaemias were very low so I
couldn't be sure and in any case the supplied protocol is obviously
designed purely for Retics. A little tinkering with your own protocol might
just do it.
If any of my old colleagues at Beckman-Coulter are monitoring the list
maybe they can help !
Wal Sharp
Lead Chief BMS Haematology
Pinderfields & Pontefract NHS Trust
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