I've been approached by an investigator who wants to separate mesophyl chloroplasts from bundle sheath chloroplasts in maize, and this is way out of my area of expertise. They gave me a reference with Bob Ashcroft's name on it, so I was hoping to get some advice from the expert! The papers were fairly old and detailed several methods including the use of differences in scatter and in fluorescence emission in the 685-710 range to isolate them. They also used ratios of fluorescence above and below 710, which seems to relate to the emission difference between chlorophylls A and B. Is this necessary (as we have no filter currently to do this) or is there another way to do this? Is anyone out there doing something similar? My second question came to mind with all of the recently discussed software issues. I have a four color XL in the lab, and one of the investigators using it wants to analyze the files with Cellquest. I saved the data in list mode, then transferred to a PC disk. My G3 could see them, but Cellquest could not. Any hints? Thanks, Carol --------------------------------------------------------------------------- Carol Oxford _| _| _|_|_|_| _|_|_| Manager, UC Davis Optical Biology Lab _| _| _| _| _| Tupper Hall, rm 3439 _| _| _| _| _| University of California _| _| _| _| _| Davis, California 95616 _| _| _| _| _| (530) 752-7205 _| _| _| _| _| (530) 752-4548 fax _|_|_|_| _|_|_|_| _|_|_| (530) 523-0967 pager cloxford@ucdavis.edu University of California, Davis ---------------------------------------------------------------------------
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