Fellow flowers- I would like to get a consensus opinion on the following: A Fellow I work with has been "doubling up" on stain (meaning mixing lymphocyte markers, either CD4 or CD8 with monocyte/CD14 markers with the SAME fluorochrome). He feels that since you gate only on the Lymphs or mono's, there is no cross-contamination or increase signal from the wrong population. Is this valid? Does anyone else do this? Is one fluorochrome (PE/FICT/PerCP) better than another? The primary reason we would like to is because we are using human cells and barely have enough to run all the various analysis we'd like. It sounds a little too good.... -- Bunny Cotleur Cleveland Clinic Foundation Neurosciences NC30 216-444-1164 ****************************************************************** When you do something, you should burn yourself completely, like a good bonfire, leaving no trace of yourself. (Shunryu Suzuki)
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