We have noticed the same thing with the "TRUCOUNT" reagents. We have noticed that when you look at the CD45 percp and SSC. Most of the cells are falling in the first decade, in another word they are negative for CD45. The CD34 PE is also very weak compared when you use 20ul or even 5ul of CD34 percp from a different bottle. I am not sure if they are diluting the CD34 so much as to keep the back ground staining very low, since it is suppose to be a Lyse no wash technique. When i add 5 ul of CD45 and CD34 in addition to the recomended volume of TRUCOUNT reagents the staining looks very nice and bright and your CD34 population stands out away from the rest of the cells. I think if they increased their concentration of the antibodies people will be able to use this reagents with crude bone marrow as well. For this reason we stopped evaluating the TRUCOUNT reagnets, specially when we are paying four times the list price. I am not sure if somebody else had also a problem with the APC Calibrite beads.. there is one particular lot number that keeps failing the FACSComp on our FACSCalibur, and so far I have not heard any response from BD in Belgium for an explanation. Faisal Rawas Flow Cytometry Lab King Faisal Hospital Riyadh, KSA ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:03 EST