I am currently running sorts using various concentrations (5million/mL - 50 million/mL) and various event rates (5K events/sec - 50K events/sec). I am also using various sample media from sterile PBS-CMF to DMEM. I am collecting samples in normal media and various concentrations of FCS. I am searching for information on ideal sample pressure, ideal flow rates, ideal sample concentrations, cell viability percentages after sorting, sort purity and possible sample preparation (what media can I pass samples through the MoFlo in without changing the stream?). Any help will be greatly appreciated. Howard Robinson
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:02 EST