more bcl-2 ab questions

From: Kushman, Mary Elizabeth (MEK0705@mail.ecu.edu)
Date: Thu Jan 06 2000 - 15:49:06 EST


I have tried to post this to the list earlier today; I don't know if it was
successful, so if there's two postings from me on this subject, I apologize
in advance.

I am interested in characterizing a population of cells which have recently
been transfected with a Bcl-2 construct.  I want to know what percentage of
cells will stain Bcl-2 positive.  My question is:  Is there a way to do this
without killing the cells?  I know that I can label my cells and sort the
positives, and I do not plan to fix sterily sorted cells.

(I know this must sound mundane but I'm still a graduate student, learning
things!)

I've been following some of the recent discussion on the Bcl-2 ab; it has
been helpful.

Any info will be greatly appreciated.  Thanks in advance!

Mary Kushman
East Carolina University



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