Dear Flowers, > Happy New Year I send before holiday this e-mail, still now I did not get any suggestion. Now I thought all come back, and send again. >I have a question about the Tunel assay using flow cytometry. I treated my >cells ( adherent cell line) with agents, Actinomysin D, Ceramide, >Campothecin, before staining with dUTP-FITC (kit from boehringer mannhein) >to detect apoptosis.What does mean when the label sample(negative sample) is >higher( histogram peak) than positive sample (TdT sample). >I would appreciate any help anyone can give.
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