Dear All, I wonder if someone out there can help me with a problem. I need to perform some experiments as part of my research to determine the sensitivity of my triple colour flow cytometry technique, which I am using for minimal residual disease monitoring. I know that the ideal is to do this on dilutions of each leukaemic sample with a normal marrow/peripheral blood sample. Unfortunately, however, limited sample quantity and time means this has not been possible. Does anyone know of a source of a leukaemic cell line with an appropriate phenotype that could be used for this purpose? (I have seen the paper by Neale et al (Leukaemia 1999, 13, pp 1221-1226) in which they used the cell line KOPN-57bi and KOR-SA3544 monoclonal antibody. Does anyone know where this cell line and reagent can be obtained?) Thanks in anticipation, Sarah Lawson (isaac.lawson@btinternet.com)
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