We already make highly luminescent beads that have europium and platinum inside them that have huge Stokes shifts and can be easily excited in the UV; however, I doubt that they (or any other long-lifetime metal-coated bead) would be very useful for flow because their emission rates are so slow that one will be lucky to get one photon out during the transit time. They have decay times of >40 microseconds for the platinum microspheres and >600 microseconds for the europium microspheres. Figure 6.45 Excitation and emission spectra of the FluoSpheres platinum luminescent microspheres (F-20886, F-20887, F-20888, F-20889, F-20890, F-20891). [Image] Figure 6.44 Fluorescence excitation and emission maxima of the FluoSpheres europium luminescent microspheres (F-20880, F-20881, F-20882, F-20883, F-20884, F-20885). [Image] Julie Nelson wrote: > Thanks so much for your quick responses to my luminescent metals > question. It looks like Plan B (attaching them to fluorescent beads) > may be the way to go. Thanks again to everyone who responded. > > Julie > > Julie G. Nelson > Research Coordinator > Flow Cytometry Facility > Center for Tropical and Emerging Global Diseases > University of Georgia
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