Flow-ers, what about packaging the unknown metal sample and a secondary/tandem dye in a liposome? Laser----> Metal(short life high conc)----->Dye (long life lower conc)-----> Data (MMMMM DATA!) Adrian ----- Original Message ----- From: "Howard Shapiro" <hms@shapirolab.com> To: cyto-inbox Cc: "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu> Sent: Thursday, March 28, 2002 3:38 PM Subject: Re: Luminescent metals > > Julie Nelson wrote- > > >I just finished talking to a researcher who has some exciting ideas > >about detecting luminescent metals using flow cytometry, specifically > >Eu, Tb and Ho. Has anyone every used flow to analyze these? Will UV > >excitation (351 nm) work? > > These rare earth metals have been used as immunufluorescent labels in > chelated form. Their fluorescent lifetimes are hundreds of microseconds, > making them incompatible with conventional commercial flow cytometers, > although they have been detected in special laboratory-built instruments. > > -Howard > > >
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