Hi Pat, There are several publications looking at this. One thing to keep in mind is that there are variables other than the PB CD34+ count that will contribute to graft collection efficiency, for example type of apheresis equipment and software version, volume of apheresis (that is a 2 blood volume apheresis procedure will produce significantly less CD34+ cells than a 4 blood volume procedure). That said, the PB CD34+ count is useful in determining whether a patient is likely to have a successful collection. We use a cut of of 5 CD34+ cells per microliter, below which the patient will not proceed to apheresis. Others use 10 or even 20 CD34+cells/uL. However this is partly due to the insensitivity of first generation CD34+ enumeration techniques to estimate very low numbers of cells. As you are using a single platform method based on the ISHAGE guidelines, you shouldn't have a problem. Feel free to contact me directly if you want specific references. Regards, Mike Keeney >>> Pat McMahon <Pat.McMahon@mckennan.org> 03/26/02 14:41 PM >>> Dear Flow folks: I am relatively new to this users group and new to flow so please bear with me. Our facility currently collects CD34+ stem cells for stem cell transplantation using apheresis. Our Apheresis collection center is interested in performing peripheral blood CD34 stem cell enumeration on patients prior to aphersis collection. The goal is to determine if a patient has an adequate number of CD34+ stem cells before performing the apheresis collection. They do not wish, necessarily, to collect the entire CD34 dose in one collection but, rather to ensure that there is adequate cd34+ stem cells such that 2-3 collections would yild an adequate dose for transplantation. Does anyone have any experience with this type of data. Are there some guidelines as to how many cd34+ stem cells /ul of peripheral blood are necessary to give an acceptable apheresis collection dosage? I am using an Epics XL and the Beckman/Coulter Stem-kit for CD34 enumerations. Thanks for any help. Patrick Patrick McMahon Avera Mckennan Hospital Sioux Falls, SD email Pat.McMahon@mckennan.org Fax: 605-322-7150
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