Dear Colleagues Some coworkers in my lab would like to count mouse monocytes in peripheral whole blood using flow cytometry. They tried this using a cytometer (in the local teaching hospital) that was setup for human blood but had no success. Is it possible to gate for murine moncytes on FSC/SSC or is a fluorochrome-labeled antibody system required? I presume for absolute and comparative enumeration it would be best to use fluorescent beads as a counting standard. Do you agree? If anyone could suggest a method or guide us to a reference paper we'd be very grateful. Best wishes Simon Watson Phagocyte Lab MRC Centre for Inflammation Research University of Edinburgh Medical School Edinburgh UK
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