Hello all, I posted a question last November about the sterility of sorts soon after we had upgraded our Vantage to an 'SE'. Thanks again to all who replied with advice. For those who may be interested, here is our solution. We started by flushing all the lines with 10% Milton. This helped but there was still contamination left in the system so we got the BD engineer to change all the sheath and sample tubing. This has solved our problems but we now fit on a new autoclaved sheath filter daily. We also use sterile PBS in the sheath tank which we change daily. If we are using the Turbosort we use a high pressure millipore sheath filter which can be autoclaved and unlike th Pall Filter, it will last indefinately. If anyone would like more information on this feel free to let me know. Regards, Mike Hughes This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute For Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
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