We have tried a lipid reagent called BioPorter (from Gene Therapy Systems) and a peptide-based reagent called Chariot (from Active Motif). Chariot was not reliable in our hands. BioPorter got antibodies into HeLa cells nicely, but they (Alexa 594 conjugated anti-IgG Ab) tended to be localized in punctate spots around the nucleus. We didn't look at antibodies that are expected to localize to specific places, but another Alexa 594-conjugated protein that we tried should have been localized to the periphery of the cell and still showed the punctate perinuclear staining. We saw clear evidence of its expected function in the periphery, however, suggesting that there is a fraction of the protein that gets to the right place despite the unexpected localization of the bulk of the protein. The data wouldn't convince a skeptic, though. Laird Bloom Phylos, Inc. 128 Spring St. Lexington, MA 02421 tel. (781) 862-6400 ext. 253 fax (781) 402-8813 www.phylos.com > ---------- > From: ray hester > Sent: Thursday, March 21, 2002 9:20 AM > To: Cytometry Mailing List > Subject: getting antibodies into viable cells > > > Greetings, > > A recent discussion on the Confocal Listserv, concerning DAPI staining of > viable cells, prompts me to send the following from a colleague: > > Is there a way to deliver antibodies, or their fragments, into viable > cells? > We have heard of using liposomes, but wondered if perhaps newer techniques > have emerged. > > Thanks. > > Ray Hester > Univ. of South Alabama > Mobile, AL 36688 > > rhester@jaguar1.usouthal.edu > > > > >
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