Wayne, There are two different approaches that might yield useful results here: 1. Injection of a cell-permea esterase substrate like Calcein AM - this should enter cells near the injection site quickly and label them for a reasonable period of time (hours). Dye that leaks out of the cells should be polar enough that it won't label cells downstream. 2. Use a lipophilic dye like DiI, which should bind to the membrane of the cells nearest the injection site. This dye will label cells for long periods, but can transfer from one cell to adjoining cells if their membranes are touching. I am sure others will have further suggestions. Best regards, Michael Kuhn, President Helix Research Fluorescence Chemistry mkuhn@helixresearch.com www.helixresearch.com ----- Original Message ----- From: Wayne Harris <waharri@emory.edu> To: cyto-inbox Sent: Thursday, March 14, 2002 10:34 AM Subject: injectable dye > > Hello Flow-ers, > > I was hoping someone out there could lead me in the right direction. I was > interested in finding an injectable fluorescent dye (into mice) that would > hopefully label cells in a concentration dependent manner so that cells > local to the site of injection would be labelled more intensely than more > distant cells. My goal is to find a way to label resident cells and track > their movement. It doesn't have to be a long term label. I'm trying to look > at peritoneal exudate cells and what happens to them upon bacterial > infection in vivo. I'm completely lost on a dye that would label preferably > in something other than FL-1 and yet would not be too toxic to be used in > such a way. Maybe someone out there has a protocol for something like this. > I would deeply appreciate any help in this endeavour. > > thanks > > > -- > Wayne Harris > Lead Research Specialist > > Department of Microbiology and Immunology > Emory University , School of Medicine > > > > >
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