Hi Howard further to your question: > And there's time (but not much) to clear this up in the 4th Edition. Do > you have a reference to Dirk van Bockstaele's calculation? > I actually have no reference for this calculation: it is just one of those quick and dirty calculations (see below) that I improvise during my lectures to students! I hope I don't miss anything important. you actually also need to know your drop drive frequency and jet velocity. Assuming a drop drive frequency of 50,000 drops/sec and a jet velocity of say 10 m/sec, you can envisage the jet as a cylindrical column of liquid with a circular base of surface pi.r2 with r = 25microm (nozzle radius) and height = 10 m (for a 1 second long flow time): total volume = pi.r.r.h. This column is broken up in 50,000 pieces (drop drive). Some quick and dirty calculation results in 1 drop = 3.93 10-4 microl or 2545 drops will sum up to a volume of 1microl, provided I didn't make any power of ten mistake! Just fill in your actual drop drive and jet velocity. KInd Regards, Looking forward to the 4-th edition! Dirk Prof. Dirk Van Bockstaele Laboratory of Hematology Head of Flow Cytometry / Molecular Diagnostics Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium phone 32 3 821 3900 fax 32 3 825 1148
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