Tom Fleisher's groups has reported flow cytometric detection of phosphorylated STAT-4 by using an anti-phospho STAT-4 antibody and intracellular staining (Uzel, Clin Imm, 100, pg. 270, 2001). I am trying to stain for phospho-STAT-5. Does anyone have any experience with this? I would be grateful to run a few questions by you in relation to choice of antibody, etc. If your help makes a substantial contribution to the resultant paper I would be happy to make you a co-author. thanks. Calman > _______________________ > Calman Prussin > Laboratory of Allergic Diseases > NIAID/ National Institutes of Health > Building 10, Room 11C205, MSC-1881 > Bethesda, MD 20892-1881 > (301) 496-1306 (Voice) > (301) 496-1306 (Fax) > calman@nih.gov
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