Dear Flowers, I have been started a new project dealing with mouse macrophages, although so far I have used in my studies only human cells. I am trying to stimulate mouse macrophages to produce IL-10 and IL-12 which I want to detect as a intracellular form by the use of flow cytometry. I am using a standard Pharmingen protocol with IFNg preactivation and subsequent LPS stimulation for 24, 48h. Unfortunately, I can detect very small (5%) amount of IL-12 positive macrophages and none of IL-10 positive. Could you give me any working protokol for intracellular detection of these cytokines in mice? Thank you in advance, Jarek Baran, Ph.D. Dept.of Pathology J.G.Brown Cancer Center University of Louisville, KY _________________________________________________________________ Chat with friends online, try MSN Messenger: http://messenger.msn.com
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